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A New Compact, Portable Gel Documentation System for Real-Time Fractionation

Cleaver Scientific Ltd
17 Jan 2013

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A new lightweight, portable gel documentation system which enables visualization of real-time size fractionation of nucleic acids has been introduced by electrophoresis specialists CLEAVER SCIENTIFIC LTD (CSL). The RunDOC gel documentation system is designed exclusively for use with CSL’s innovative runVIEW system and comprises a darkroom hood and a 10 megapixel digital camera to capture images of nucleic acids gels stained with EtBr, SYBR and runSAFE.

RunDOC is designed to be placed directly over the RVMSCHOICE gel tank located on the runVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED gel illuminator provides the excitation source for the DNA gel located within the tank and following excitation the light emitted is then visualized using either one of the bluVIEW lid options supplied with runVIEW, or the runDOC filter slide.

In addition to being lightweight runDOC has a small footprint and so requires only minimal space within the laboratory. Once fitted quickly and easily over the gel tank and base unit, gels can be imaged either in real-time or at the end of the electrophoresis run. Integral extractor fans in the bluVIEW lid and the runDOC darkroom hood remove any condensation to ensure that visualization and resolution are not impaired, during and after electrophoresis.

RunDOC’s high resolution 10 megapixel camera with 4GB memory card allows for storage of images in either RAW or JPEG formats, while the interchangeable filter slide and bluVIEW filter options provide the user with optimum flexibility.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Gel ImagingGel imaging captures high-resolution images of electrophoresis gels to analyze DNA, RNA, and proteins. Essential in molecular biology, this technique helps visualize results for accurate quantification and documentation. Discover top imaging systems and compare user-reviewed options in our directory.

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