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3D Cell Culture Methods and Protocols

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From 3D Culture Systems to Spheroids – break into three-dimensional (3D) cell culture with the latest methods and resources

10 Jul 2019
Matthew Mcardle

Editorial article

3D Cell Culture Technology

This month, we showcase some of the latest technology and guidance in the rapidly expanding world of three-dimensional (3D) cell culture. In this highlights article, learn about the newest developments in 3D cell culture and biology, including advanced computational clearing technology,  laser confocal microscopy, and magnetic 3D bioprinting; and check out our pick of some of the best videos and resources in the field.

Visit our 3D cell culture special feature, or read on for:

Hot Topics in 3D Cell Culture:

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The power of advanced computational clearing technology and THUNDER imaging systems. Image by Dr. Jan Shumacher, Leica Microsystems, Germany

Trends in 3D Cell Culture:

Image of the CosmosID stacked bar graph

Explore our selection of the biggest news stories in cell biology:

Article: Technology Trends: Cancer Research

Article: Modeling Kidney Disease with Bioengineered Kidney Organoids

Application eBook: Optical Sectioning Solutions for 3D Cancer Cell Analysis

Application eBook: Live-Cell Analysis: From Culture to Assay

Application Note: Thunder Imagers Decode 3D Biology in Real Time

Video: Vascularization of 3D Tissues In Vitro with the OrganoPlate Graft

Top 3D Cell Culture Product Reviews

Read reviews left by scientists to help you choose the best instruments for your lab, or share your opinion to help your peers by writing your own review today. Read one of our top 3D cell culture product reviews for the VersaGel®/ Symphony® 3D cell culture platform by Cypre Inc. and help make your next buying decision easier:

3D Cell Culture Special Feature Review

Explore Scientific Innovation on The Scientists’ Channel

Discover leading scientists on video:

Visit the SelectScience 3D cell culture special feature to find out more about the latest research and technology advancing the field>>

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.

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